Mes buffer

N-Morpholino)ethansulfonsäure, auch als MES abgekürzt, ist eine in den Biowissenschaften häufig benutzte synthetisch hergestellte Puffersubstanz aus der Gruppe der Good-Puffer. Der pKa-Wert von MES ist (°C), der Pufferbereich beträgt pH 8–5. MES ist strukturell eng verwandt mit MOPS, welches das . MES is the common name for the compound 2-(N-morpholino)ethanesulfonic acid.

Its chemical structure contains a morpholine ring. It has a molecular weight of 195.

Induction of polymerization of purified tubulin by sulfonate buffers. Marked differences between 4-morpholineethanesulfonate ( Mes ) and 4- piperazineethanesulfonate (Pipes). Interactions of both purified tubulin and microtubule . MES it is not absorbed through cell membranes, and it is essentially transparent to UV light.

MES is used as a running buffer for resolving very small proteins (ex: kDa) on bis-tris gels. It was used to prepare buffer to study the expression profiling by microarrays. MES sodium salt is suitable for cell culture and diagnostic assay manufacturing. Assay ‎: ‎≥ (titration) PKa ‎: ‎6.

What is the protocol of MES buffer preparation?

Seiten Diese Seite übersetzen Get expert to your questions in Buffer Preparation and more on ResearchGate, the professional network for scientists. Description: MES (2-(N-morpholino) ethanesulfonic acid) Buffer is a synthetic buffer and is used in plant cell cultures. MES Buffer has a buffering range from pH 5. Molecular Biology, ultra pure grade . NuPAGE MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Novex Bis-Tris gels only. Adjust to desired pH using 10N NaOH.

Filter sterilize (recommended) or autoclave. Low absorption spectra in UV and visible regions. Ka (25°C) pKa (37°C) cat. Making MES is the same as making most any buffer. Essentially you titrate a fixed amount of MES in either its acid or basic form.

For MES the starting point is usually the free acid which you titrate with NaOH to the correct pH. Definition: A buffer solution where the pH is maintained by an equilibrium involving 2-(N-morpholino)ethanesulfonic acid. Synonyms: MES buffer solution. More about the RSC Chemical Methods Ontology (CMO) . Solutions are stable at 2-°C for months.

Autoclaving is not recommended for any sulfonic acid buffer. When MES solutions are autoclave they turn yellow ( although pH does not change measurably).

The identity of the yellow breakdown product is unknown. Boston Bioproducts Inc Supplier Diversity Partner MES - BUFFER. Find this and many more products.

M 2-(N-morpholino)ethanesulphonic aci 0. M phosphate, mM EDTA, pH 6. Warning - this product is not for human or veterinary use.